Latex agglutination test Kit to Detect Swine Antibody against Japanese encephalitis Reagents                                                            

                                                                                Volume
- Latex antigen                                                       2 ml
- Positive control                                                   1 ml
- Negative control                                                  1 ml
- Diluting buffer                                                      16 ml  

Test Protocol and Result

Qualitative test:
Each drop (20μl) of the tested sample (serum or whole blood), positive serum, negative serum and diluting buffer is placed on glass and mixed with one drop (20μl) of latex antigen. The mixture is stirred for 1 to 2 min and the result is observed and determined within 3 to 5 min.

Quantitative test:
The tested sample is diluted in series by 2-fold in a micro reaction plate or EP tube. Each drop (20μl) of the diluted sample is placed on glass, with negative and positive serum as controls. Then one drop (20μl) of latex antigen is added to each sample and the mixture is stirred as described above. The highest dilution fold that realizes a positive agglutination reaction is decided as the antibody potency of the tested serum.

The standard of agglutination intensity
“++++”: All the latex is agglutinated, and the particles accumulated at the edge of the drop. The liquid is totally transparent.
“+++”: Most of the latex is agglutinated. The particles are obvious and the liquid is a little bit opaque.
 “++”: Half of the latex is agglutinated. The particles are thin and the liquid is opaque.  
 “+”:  Little is agglutinated and the liquid is rather opaque. 
 “-”: The drop remains the homogenized emulsion as before.

When a reaction stronger than “++” is shown, the serum is determined as positive.

Storage    Tests must be storaged at 2~8℃.